Evelyn Wong
Funded by the British Society for Surgery of the Hand
Project Title: An in-vitro laser axotomy model for peripheral nerve injury: investigating Golgi apparatus and cytoskeletal dynamics in adult dorsal root ganglion neurons
Supervisor: Dr Raman Das and Mr Adam Reid
Institution: University of Manchester
Peripheral Nerve Injuries (PNIs) affect how the brain communicates with muscles in our arms, hands, legs, and feet. They are common: in the UK, it's estimated almost 1 in 10 people aged 55 or over are affected by PNIs.
Despite surgical repair, most people never recover fully. To understand more about how we can treat these injuries, we need to understand more about the regenerative abilities of damaged nerves.
The project will use a special laser to injure neurons (from dorsal ganglion cells), mimicking the damage done by PNIs. By using this laser, the team will be able to observe how nerve cells heal - in particular looking at the Golgi and cytoskeletal apparatus of the cell changes.
By the end of the project, we will know more about how nerve cells heal, what influences the rate of healing, and how the Golgi Apparatus changes during healing. There will also be a new robust framework established for duplicating PNIs in single cells, which could open the door to many new studies in various fields.
I am very grateful to Scar Free and BSSH for this unique opportunity to conduct my research elective at the University of Manchester. My elective is on a topic I am deeply passionate about - investigating peripheral nerve regeneration with the goal of supporting the repair process for patients after traumatic nerve injuries. I hope my work will uncover valuable insights about the intracellular regenerative mechanisms which can lead to development of novel therapies to improve nerve repair outcomes.